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Separation & Purification of an Invertase and an Inulase from Germinating Garlic (Allium sativum L) Bulbs

Bhat, Gopalakrishna P and Pattabiraman, TN (1980) Separation & Purification of an Invertase and an Inulase from Germinating Garlic (Allium sativum L) Bulbs. Indian Journal of Biochemistry & Biophysics, 17. pp. 338-343.

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Abstract

An acid invertase (pH optimum 4.6) and an inulase (pH optimum 5.0) were separated. purified and charactcrizcd from germinating garlic bulbs. Both the enzymcs had apparent molecular weights or 76.0QO daltons as determined by gel chromatography OD SephadeJ< G-200 and were inactivated by treatmenl with p-<:hloromercuribcnzoate. 5.5'-dithiobis (2- nitrobcnzoic acld) and heat treatment at 55'C for 5 min at pH 7.0. The invertase hydrolyzed sucrose and raffinosc but had no action on inulin. The apparent Km value for sucrose was 26 mM. The inulase hydrolyzed. apart from inulin, sucrose and raffinose. The K~ values for inulin and sucrose were 10 mM and 25 mM' respectively. While in the early slages or plant growth (up to 6 days) the inulase and invertase activities incrcased in a parallel fashion in the bulbs. at Ialer stages Ihe iDf;rease in invertase activity "'as more than that or inulase activity.

Item Type: Article
Subjects: Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 08 Dec 2011 05:54
Last Modified: 08 Dec 2011 05:54
URI: http://eprints.manipal.edu/id/eprint/1786

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