Expression of the Basement Membrane Anchoring Fibril Component Type VII Collagen in Different Grades of Oral Squamous Cell Carcinoma – An Immunohistochemical Study

Sudhir, * and Rao, Nirmala N and Kotian, MS (2009) Expression of the Basement Membrane Anchoring Fibril Component Type VII Collagen in Different Grades of Oral Squamous Cell Carcinoma – An Immunohistochemical Study. Malaysian Dental Journal, 30 (2). pp. 100-104.

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Abstract

Basement membranes are involved in a number of biological processes and act as a three dimensional supporting scaffold. They form an important barrier against tumour cell invasion. Recently, biochemical characterization and production of antibodies against different constituents of basement membrane have proved to be more reliable in tumour invasion and metastasis. Objective-The present study aimed to assess the role of type VII collagen in oral squamous cell carcinoma samples. A principle constituent of anchoring fibril which link the basal lamina of oral mucosa to the underlying connective tissue. We also observed the intensity and location of type VII collagen immuno-histochemically, and to consider this marker as a useful adjunct in grading and to identify more potentially aggressive and invasive margins in the lesion. Material and method- Forty-two samples of oral squamous cell carcinoma and seven controls from buccal mucosa were stained with mouse anti human anti collagen type VII, at a dilution of 1:1000, visualized by DAB substrate. The antigen positive areas with varied intensity of staining were then assessed in all the samples. Results- The deposition of type VII collagen antigen was evident around and within the malignant cells. Statistical evaluation of data by using Chi square test and Fischer exact test revealed very highly significant relationship in localization and intensity of staining of this antigen and histpathological grades of OSCC. The control tissues demonstrated a deposition of this antigen at the level of basement membrane. Conclusion- In conclusion the tumour cells exhibited an altered synthesis and secretion of type VII collagen protein. Further as the degree of de –differentiation in tumour cells increased there was an observable lack of extra cellular protein secretion.

Item Type: Article
Uncontrolled Keywords: Oral Squamous cell carcinoma, basement membrane, Type Vll collagen fibers, Immunohistochemical
Subjects: Dentistry > MCODS Manipal > Oral Pathology and Microbiology
Depositing User: KMC Manipal
Date Deposited: 22 Dec 2011 07:05
Last Modified: 22 Dec 2011 07:05
URI: http://eprints.manipal.edu/id/eprint/1901

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