An indirect immunofluorescence study in different grades of oral squamous cell carcinoma

Prakash, S and Rao, MN and Kotian, MN (2008) An indirect immunofluorescence study in different grades of oral squamous cell carcinoma. Malaysian Dental Journal , 29 (1). pp. 51-54.

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Abstract

It is well known that malignant cells may exhibit profound alteration on their cell surface proteins, which can belocalized by employing certain markers either by immunofluorescence or by immunohistochemistry. These markersare of great value in establishing the diagnosis and predicting the true biological potential more accurately thanconventional histology. Objective: In the present study an attempt has been made to demonstrate the expressionof pemphigus vulgaris antigen (PVA) in different histological grades of oral squamous cell carcinoma. Materialsand methods: Tissue sections from twenty five clinically and histo-pathologically diagnosed oral squamous cellcarcinoma and five normal buccal mucosa as controls were stained with pemphigus serum collected from untreatedcases of pemphigus vulgaris. These were subsequently stained with fluorescein-labeled rabbit anti human IgG.Results: Deposition of IgG was evident in different grades of Oral Squamour Cell Cascinoma (OSCC) in varyingintensities at inter cellular adhesion junctions. Statistical evaluation of data by using χ2 test- Yates correction forcontinuity revealed very highly significant (p < 0.001; χ2 = 28.355 test-Yates correction for continuity) relationshipin the expression of this antigen and histo-pathological grades of OSCC. The control specimens in the studydemonstrated the retention of PV antigen at intercellular spaces. Conclusions: The results of the study showeda significant reduction in PVA expression at invasive sites more so in poorly differentiated carcinoma. Thus analtered expression (retention or absence) in the cell surface antigen suggest a basis to develop an approach inearly diagnosis of primary OSCC. Also it can be viewed that antigen deletion may be an important factor in localinvasion by malignant cells.

Item Type: Article
Uncontrolled Keywords: Indirect immunofluorescence study, oral squamos cell carcinoma
Subjects: Dentistry > MCODS Manipal > Oral Pathology and Microbiology
Depositing User: KMC Manipal
Date Deposited: 28 Dec 2011 11:10
Last Modified: 28 Dec 2011 11:10
URI: http://eprints.manipal.edu/id/eprint/1984

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