Singh, R Eshwar and *, KG Raghukumar and *, M Veena and *, G Vishwanath and Rao, PN Sridhar and *, BV Murlimanju (2012) ESBL Production: Resistance Pattern in Escherichia coli and Klebsiella pneumoniae, A Study by NCCLS method. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 3 (1). pp. 559-565. ISSN 0975-8585

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Abstract

Various factors are involved in resistance to third generation cephalosporins by E. coli and K. pneumoniae. The objectives of present study were to study the resistance pattern due to ESBL’s. The study comprised a total of 200 clinical specimens which included urine, pus, sputum, blood, throat swabs, suction tips and vaginal swabs. E. coli and K. pneumoniae isolates were studied for ESBL production by NCCLS method. Culture of 200 samples yielded 117 E. coli and 83 K. pneumoniae isolates. Among them, 98 (49%) were screen positive for ESBL. Among them 53 E. coli and 26 K. pneumoniae were found to produce ESBL. In that, 52 isolates of E. coli and 26 isolates K. pneumoniae were positive by NCCLS-PCT method. The present study observed ESBL production in 39.5% of cases. All the ESBL isolates were susceptible to imipenem, however 98.1% cases were resistant to cefoxitin. The present study observed higher prevalence of ESBL producing E. coli and K. pneumoniae. We suggest that routine screening of ESBL should be performed on all isolates which are showing decreased susceptibility to third generation cephalosporins.

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