AmpC Beta Lactamases Among ESBL Producing Escherichia Coli And Klebsiella Pneumoniae- If You Don’t Look, You Won’t Find

Vandana, KE and Honnavar, P (2009) AmpC Beta Lactamases Among ESBL Producing Escherichia Coli And Klebsiella Pneumoniae- If You Don’t Look, You Won’t Find. Journal of Clinical and Diagnostic Research, 3 (4). pp. 1653-1656.

[img] PDF
AmpC_Beta_Lactamases_Among_ESBL_Producing.pdf - Published Version
Restricted to Registered users only

Download (300kB) | Request a copy

Abstract

Amp C beta lactamases confer resistance to a wide variety of beta lactams and pose both diagnostic and therapeutic challenges, as their presence goes undetected in the presence of ESBLs. We evaluated 52 ESBL positive clinical isolates of E.coli (n=41) and K.pneumoniae (n=11) for AmpC production by a phenyl boronic acid disc (PBA) and the 3 dimensional enzyme test (3DET). The PBA method detected 24 (58.5%) and 9 (82%)E.coli and K.pneumoniae isolates as AmpC positive, while detection by the 3DET increased the numbers to 29 (70.7%) and 10 (91%) isolates, respectively. We conclude that a large number (75%) of ESBL producers are also found to produce AmpC and that the PBA disc method is a very useful and reliable method for routine use in laboratories.

Item Type: Article
Uncontrolled Keywords: Amp C beta lacatamase, ESBL, phenyl boronic acid, 3 dimensional enzyme test
Subjects: Medicine > KMC Manipal > Nephrology
Depositing User: KMC Manipal
Date Deposited: 11 Jun 2011 10:45
Last Modified: 11 Jun 2011 10:45
URI: http://eprints.manipal.edu/id/eprint/247

Actions (login required)

View Item View Item