Acute diuretic activity of alcoholic extracts of hygrophila auriculata seeds in normal Wistar Albino rats

Preethi, GP and Gopalakrishna, HN and Rathnakar, UP and Durga, P and Vishnu, * and Shenoy, Jnaneshwara (2012) Acute diuretic activity of alcoholic extracts of hygrophila auriculata seeds in normal Wistar Albino rats. International Journal of Pharma and Bio Sciences, 3 (1). pp. 283-289. ISSN 0975-6299

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Abstract

Objectives: The present study was undertaken to investigate the diuretic properties of the seeds of Hygrophila auriculata (Schum) (Acanthaceae) in normal Wistar Albino rats. Methods: Alcohol extracts of seeds of H. auriculata (300mg/kg and 500mg/kg p.o.) were investigated for diuretic activity by measuring the total urine output over 24hrs and electrolytes (Sodium, Potassium and Chloride) estimation in Wistar rats (n=6). Frusemide (20 mg/kg, p.o), a high ceiling diuretic served as positive control and normal saline (25 ml/kg, p.o) as placebo control. Results: The urine volume was 3.33 ±0.13ml, 9.12 ± 0.25 ml, 3.1833 ± 0.15ml and 5.52 ±0.18 ml for control, frusemide, extract at 300mg/kg and 500mg/kg dose respectively. There was significant increase in sodium (198.33 ± 2.99 m.mol/L), potassium (97.67 ± 2.33 m.mol/L) and chloride ion excretion (132.67 ± 2.65 m.mol/L) in the 500mg/kg alcohol extract as compared to control group (107 ± 2.11 m.mol/L, 55 ± 4.09 m.mol/L & 87.33 ± 2.33 m.mol/L respectively) (P < 0.001). Conclusion: The alcoholic extract of H.auriculata showed significant diuretic properties. At both the doses employed there was a significant increase in electrolyte excretions, however increase in urine volume was significant only at 500mg/kg. Further studies elucidating mechanism of action and employing lower doses are warranted.

Item Type: Article
Uncontrolled Keywords: diuretic index, Lipschitz method, saluretic index
Subjects: Medicine > KMC Mangalore > Pharmacology
Depositing User: KMCMLR User
Date Deposited: 14 Jan 2012 04:20
Last Modified: 14 Jan 2012 04:20
URI: http://eprints.manipal.edu/id/eprint/2486

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