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High Performance Liquid Chromatographic Fluorescence Detection Method for the Quantification of Rivastigmine in Rat Plasma and Brain: Application to Preclinical Pharmacokinetic Studies in Rats.

Arumugam, K and Chamallamudi, MR and Surulivelrajan, Mallayasamy and Mullangi, R and Ganesan, S and Jamadar, L and Ranjithkumar, A and Udupa, N (2011) High Performance Liquid Chromatographic Fluorescence Detection Method for the Quantification of Rivastigmine in Rat Plasma and Brain: Application to Preclinical Pharmacokinetic Studies in Rats. Journal of Young Pharmacists, 3 (4). pp. 315-321. ISSN 0975-1483

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Abstract

A highly sensitive and selective high performance liquid chromatographic fluorescence detection method has been developed and validated for the quantification of rivastigmine in rat plasma and brain. Protein precipitation and one‑step liquid–liquid extraction techniques were utilized for the extraction of RSM from brain and plasma, respectively, along with an internal standard. The chromatographic separation was achieved with a column inertsil ODS‑3V and a mobile phase consisting of ammonium acetate buffer (20 mM, pH 4.5) and acetonitrile (76:24, v/v) delivered at a flow rate of 1 ml/min. The lower limit of quantitation for the developed method was 10 ng/mL for both matrices. The method was found to be accurate and reproducible and was successfully used to quantify levels of RSM in plasma and brain following intravenous administration of RSM in rats.

Item Type: Article
Uncontrolled Keywords: HPLC method validation, pharmacokinetics, plasma-brain correlation, rivastigmine
Subjects: Pharmacy > MCOPS Manipal > Pharmacy Management
Depositing User: KMC Manipal
Date Deposited: 18 Jan 2012 04:50
Last Modified: 28 Jun 2012 06:40
URI: http://eprints.manipal.edu/id/eprint/2605

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