Phenolic compounds isolated from Pilea microphylla prevent radiation-induced cellular DNA damage

Bansal, Punit and Paul, Piya and Nayak, Pawan G and Pannakal, Steve T and Zou, Jian-hua and Laatsch, Hartmut and Priyadarsin, KI and Unnikrishnan, MK (2011) Phenolic compounds isolated from Pilea microphylla prevent radiation-induced cellular DNA damage. Acta Pharmaceutica Sinica B, 1 (4). pp. 226-235. ISSN 2211-3835

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Six phenolic compounds namely,quercetin-3-O-rutinoside (1), 3-O-caffeoylquinic acid (2), luteolin-7-O-glucoside (3), apigenin-7-O-rutinoside (4), apigenin-7-O-b-D-glucopyranoside (5) and quercetin (6) were isolated from the whole plant of Pilea microphylla using conventional open- silica gel column chromatography and preparative HPLC.Further,these compounds were characterized by 1D,2DNMRtechniques and high-resolution LC–MS.Compounds 1–3 and 6 exhibited significantant aioxidant potential in scavenging free radicals such as DPPH, ABTS and SOD with IC50 of 3.3–20.4 mmol/L. The same compounds also prevented lipid peroxidation with IC50 of 10.4–32.2 mmol/L. The compounds also significant ly prevented the Fenton reagent-induced calf thymusDNAdamage.Pre-treatment with compounds 1–3 and 6 in V79 cell sattenuated radiation-induced formation of reactive oxygen species,lipid peroxidation,cyto toxicity and DNA damage, correlating the antioxidant activity of polyphenols with the irradio protective effects. Compounds 1, 3 and 6 significantly in hibited lipid peroxidation,presumably due to 30,40-catechol ortho-dihydroxy moietyintheB-ring,which has a strong affinity for phospholipid membranes. Oxidation offlavonoids,with catechol structureon B ring,yields a fairly stable ortho-semiquinone

Item Type: Article
Uncontrolled Keywords: Pilea microphylla; Radioprotection; Phenolic compounds; DNA damage; Comet assay
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Pharmacy > MCOPS Manipal > Pharmacy Practice
Depositing User: KMC Manipal
Date Deposited: 19 Jan 2012 07:03
Last Modified: 19 Jan 2012 07:03

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