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Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats

Bankar, Girish R and Nayak, Pawan G and Bansal, Punit and Paul, Piya and Pai, KSR and Singl, Rajeev K and Bhat, Varadaraj G (2011) Vasorelaxant and antihypertensive effect of Cocos nucifera Linn. endocarp on isolated rat thoracic aorta and DOCA salt-induced hypertensive rats. Journal of Ethnopharmacology, 134. pp. 50-54. ISSN 0378-8741

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Abstract

Ethnopharmacological relevance: The fruits of Cocos nucifera Linn. (Arecaceae) have long been used in traditional medicine for the treatment of cardio-metabolic disorders. Aim of the study: To evaluate the ethanolic extract of Cocos nucifera Linn. endocarp (CNE) for its vasorelaxant activity on isolated rat aortic rings and antihypertensive effects in deoxycorticosterone acetate (DOCA) salt-induced hypertensive rats. Materials and methods: Cocos nucifera Linn. endocarp was extracted with ethanol and characterized by HPLC. CNE was examined for its in vitro vascular relaxant effects in isolated norepinephrine, phenylephrine or potassium chloride pre-contracted aortic rings (both intact endothelium and denuded). In vivo anti-hypertensive studies were conducted in DOCA salt-induced uninephrectomized male Wistar rats. Results: Removal of endothelium or pretreatment of aortic rings (intact endothelium) with l-NNA (10�M) or ODQ (10�M) followed by addition of contractile agonists prior to CNE significantly blocked the CNEinduced relaxation. Indomethacin (10�M) and atropine (1�M) partially blocked the relaxation, whereas glibenclamide (10�M)did not alter it.CNEsignificantly reduced themeansystolic blood pressure inDOCA salt-induced hypertensive rats (from 185.3±4.7mmHg to 145.6±6.1 mmHg). The activities observed were supported by the polyphenols, viz. chlorogenic acid, vanillic acid and ferulic acid identified in the extract. Conclusions: These findings reveal that the vasorelaxant and antihypertensive effects of CNE, through nitric oxide production in a concentration and endothelium-dependent manner, is due to direct activation of nitric oxide/ guanylate cyclase pathway, stimulation of muscarinic receptors and/or via cyclooxygenase pathway.

Item Type: Article
Uncontrolled Keywords: Cocos nucifera , DOCA salt-induced hypertension , Vasorelaxation , Aortic rings , Nitric oxide ,Endothelium
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 21 Jan 2012 04:34
Last Modified: 21 Jan 2012 04:34
URI: http://eprints.manipal.edu/id/eprint/2631

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