Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against �-radiation-induced cytotoxicity and genotoxicity

Paul, Piya and Bansal, Punit and Nayak, Pawan G and Pannakal, Steve Thomas and Priyadarsini, KI and Unnikrishnan, MK (2012) Polyphenolic fraction of Pilea microphylla (L.) protects Chinese hamster lung fibroblasts against �-radiation-induced cytotoxicity and genotoxicity. Environmenal Toxicology and Pharmacology, 33. pp. 107-119. ISSN 1382-6689

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Abstract

Present study was designed to compare cytoprotective and antigenotoxic activity of the polyphenolic fraction of Pilea microphylla (PM1) with that of its active polyphenolic constituents against �-radiation in V79 cells. PM1 was standardized with respect to the polyphenols present by RP-HPLC. It was evaluated for its free radical scavenging potential using Fenton reaction-induced DNA damage and lipid peroxidation. Further, PM1 was subjected against �-radiation-induced cytotoxicity and genotoxicity in V79 cells. PM1 significantly reduced free radical-mediated calf thymus DNA damage and lipid peroxidation. Among the concentrations tested (12.5, 25 and 50 �g/ml) for radioprotection, PM1 at 25 �g/ml exhibited maximum protection. Further, when compared with constituent polyphenols viz., rutin, quercetin and chlorogenic acid (concentrations equivalent to that present in PM1-25 �g/ml), a combination of polyphenols was found most effective in preventing �-radiation-induced cytotoxicity and genotoxicity. To conclude, radioprotection is possibly a synergistic effect of the phytochemicals present in the herbal extract, rather than any single component.

Item Type: Article
Uncontrolled Keywords: Pilea microphylla , Cytotoxicity , Genotoxicity , �-Radiation ,Polyphenols.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmacology
Pharmacy > MCOPS Manipal > Pharmacy Practice
Depositing User: KMC Manipal
Date Deposited: 21 Jan 2012 05:03
Last Modified: 21 Jan 2012 05:03
URI: http://eprints.manipal.edu/id/eprint/2644

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