Effect lecithin and silymarin on d-galactosamine induced toxicity in isolated hepatocytes and rats

Raj, PV and Nitesh, K and Chandrashekhar, HR and Rao, Mallikarjuna C and Rao, Venkata J and Udupa, N (2010) Effect lecithin and silymarin on d-galactosamine induced toxicity in isolated hepatocytes and rats. Indian Journal of Clinical Biochemistry, 25 (5). pp. 169-174.

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Abstract

To investigate Lecithin for its hepatoprotective activity against D-galactosamine (D-GalN) induced toxicity in freshly isolated rat hepatocytes and animal models. Freshly isolated rat hepatocytes were exposed to Dgalactosamine (30 mM) along with/without lecithin (100 μg/ml) and the levels of selected liver enzymes were measured. Thirty six Wistar strain albino rats were used for the in vivo investigations. Lecithin 50 and 100 mg/ kg.b.wt were administered for one week by oral route. Liver damage was induced by intra peritoneal administration of 400 mg/kg b.wt D-galactosamine. The antihepatotoxic effect of lecithin was observed in freshly isolated rat hepatocytes at concentration 100 μg/ml and was found to be similar to that of the standard silymarin used. Its in vivo hepatoprotective effect at 100 mg/kg b.wt was comparable with that of the standard silymarin at 100 mg/kg body weight. Lecithin was able to normalise the biochemical levels which were altered due to D-galactosamine intoxication in freshly isolated rat hepatocytes and also in animal models.

Item Type: Article
Uncontrolled Keywords: Lecithin, Silymarin, D-galactosamine, Hepatoprotective activity, Hepatocytes, Animal models.
Subjects: Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 27 Jan 2012 10:13
Last Modified: 27 Jan 2012 10:13
URI: http://eprints.manipal.edu/id/eprint/2718

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