Synthesis, antitumor, and DNA binding behavior of novel4-(2-hydroxyquinolin-3-yl)-6-phenyl-5, 6 dihydropyrimidin derivatives in aqueous medium

Lamani, Devappa S and Reddy, Venugopala KR and Naik, Bhojya HS and Pai, KSR and Kumar, Ravishankar and Fasiulla, * and Naik, Prakash HR and Naik, LR (2010) Synthesis, antitumor, and DNA binding behavior of novel4-(2-hydroxyquinolin-3-yl)-6-phenyl-5, 6 dihydropyrimidin derivatives in aqueous medium. Nucleosides, Nucleotides and Nucleic Acids, 29. pp. 591-605.

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Abstract

This article deals with the synthesis of 4-(2-hydroxyquinolin-3-yl)-6-phenyl-5,6- dihydropyrimidin derivatives (2a–f), on condensation with various aromatic aldehydes and ketones in aqueous ethanolic NaOH solution yielding the corresponding chalcones (3). These chalcones were further reacted with thiourea/urea in the presence of a base, which led to the formation of the titled derivatives (2a–f). The newly synthesized heterocyles were characterized by elemental analysis, FTIR, 1HNMR, and electronic and mass spectral data. The compounds (2a and 2b) were evulated for in vitro cyctotoxicity against human breast adenocarcinoma cell (MCF-7). In MTT cytotoxicity studies, both quinolinde derivatives were found most effective.The binding interaction behavior of the compound (2a) and (2d) with calf thymus-DNA (CT-DNA) was studied by electronic spectra, viscosity measurements, and thermal denaturation studies. On binding to CT-DNA, the absorption spectrum underwent bathochromic and hypochromic shifts. The binding constant (Kb) observed 4.3 × 105 M−1 for (2a), and 3.8 × 105 M−1 for (2d) suggested that compound (2a) binds more strongly with base pairs than (2d).

Item Type: Article
Uncontrolled Keywords: Claisen Schmidt condensation; antitumor activity; MTT assay, DNA binding; viscosity measurements; aqueous medium
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 27 Jan 2012 10:41
Last Modified: 27 Jan 2012 10:41
URI: http://eprints.manipal.edu/id/eprint/2729

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