In vitro and in hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves

Raj, Vasanth P and Chandrasekhar, Raghu H and Dhanaraj, SA and Rao, Mallikarjuna C and Rao, Venkata J and Nitesh, K (2010) In vitro and in hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves. Indian journal of pharmacology, 42 (2). pp. 98-103.

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Objective: To investigate the total alkaloid fraction of the methanol extract of leaves of Hygrophila auriculata for its hepatoprotective activity against CCl4-induced toxicity in freshly isolated rat hepatocytes, HepG2 cells, and animal models.Materials and Methods:Mature leaves of H. auriculata were collected, authenticated, and subjected to methanolic extraction followed by isolation of total alkaloid fraction. Freshly isolated rat hepatocytes were exposed to CCl4 (1%) along with/without various concentrations of the total alkaloid fraction (80–40 µg/ml). Protection of human liver-derived HepG2 cells against CCl4-induced damage was determined by the MTT assay. Twenty-four healthy Wistar albino rats (150–200 g) of either sex were used for the in vivo investigations. Liver damage was induced by administration of 30% CCl4 suspended in olive oil (1 ml/kg body weight, i.p). Results: The antihepatotoxic effect of the total alkaloid fraction was observed in freshly isolated rat hepatocytes at very low concentrations (80–40 µg/ml). A dose-dependent increase in the percentage viability was observed when CCl4-exposed HepG2 cells were treated with different concentrations of the total alkaloid fraction. Its in vivo hepatoprotective effect at 80 mg/kg body weight was comparable with that of the standard Silymarin at 250 mg/kg body weight.Conclusion:The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.

Item Type: Article
Uncontrolled Keywords: HepG2, hepatocytes, hepatoprotective, Hygrophila auriculata, total alkaloidsIntroduction
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 30 Jan 2012 06:45
Last Modified: 30 Jan 2012 06:45

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