Effects of Mutagens On Somatic Embryogenesis And Plant Regeneration In Groundnut

Muthusamy, A and Vasanth, K and Sivasankari, D and Chandrasekar, BR and Jayabalan, N (2007) Effects of Mutagens On Somatic Embryogenesis And Plant Regeneration In Groundnut. Biologia Plantarum, 51 (3). pp. 430-435.

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The embryogenic calli (EC) were obtained from hypocotyl explants of groundnut (Arachis hypogaea L.) cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) in combination with 0.5 mg dm-3 6-benzylaminopurine (BAP). The EC were exposed to γ-radiation (10 - 50 Gy) or treated with 1 - 5 mM of ethyl methane sulphonate (EMS) or sodium azide (SA). The mutated EC were subcultured on embryo induction medium containing 20 mg dm-3 2,4-D. Somatic embryos (SE) developed from these calli were transferred to MS medium supplemented with BAP (2.0 mg dm-3) and 0.5 mg dm-3 2,4-D for maturation. The welldeveloped embryos were cultured on germination medium consisting of MS salts with 2.0 mg dm-3 BAP and 0.25 mg dm-3 naphthaleneacetic acid (NAA). Well-developed plantlets were transferred for hardening and hardened plants produced normal flowers and set viable seeds. The fresh mass of the EC, mean number of SE per explant and regeneration percentage were higher at lower concentrations of mutagens (up to 30 Gy/3 mM). Some abnormalities in regenerated plants were observed, especially variations in leaf shape.

Item Type: Article
Uncontrolled Keywords: Arachis hypogaea, Chemical mutagens, Embryogenic callus, γ-Radiation, Growth regulators
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 01 Feb 2012 06:33
Last Modified: 01 Feb 2012 06:33
URI: http://eprints.manipal.edu/id/eprint/2815

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