Kamath, Sudha D and Kartha, VB and Mahato, KK (2008) Dynamics of l-tryptophan in aqueous solution by simultaneous laser induced fluorescence (LIF) and photoacoustic spectroscopy (PAS). Spectrochimica Acta Part A, 70. pp. 187-194. ISSN 1386-1425
![]() |
PDF
Spectro_Chim_Acta_Tryptophan.pdf - Published Version Restricted to Registered users only Download (569kB) | Request a copy |
Abstract
An experimental system for measuring simultaneously photoacoustic (PA) and fluorescence signals is described. The simultaneous measurement of laser induced fluorescence and photoacoustic signals provide a suitable method for the study of different quenching phenomena occurring in fluorescent systems. In this paper we report tryptophan solvation dynamics in water using fluorescence and photoacoustic spectra recorded simultaneously by photoacoustic and fluorescence signals as functions of concentration, indicate that quantum yield is maximum at low concentrations. Also, the energy lost in the fluorescence path of tryptophan, due to different quenching phenomena like self quenching, Resonance energy transfer (RET), solvation relaxation, etc. is clearly seen from the photoacoustic signal intensity which increases as the fluorescence intensity decreases.
Item Type: | Article |
---|---|
Uncontrolled Keywords: | l-Tryptophan; Photoacoustics; Fluorescence; Quantum yield; Quenching |
Subjects: | Life Sciences > MLSC Manipal |
Depositing User: | KMC Manipal |
Date Deposited: | 08 Feb 2012 10:32 |
Last Modified: | 07 Oct 2013 06:52 |
URI: | http://eprints.manipal.edu/id/eprint/2907 |
Actions (login required)
![]() |
View Item |