Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmen tal period: a morph ometric analysis

Potu, Bhagath Kumar and Rao, Muddanna S and Kutty, Gopalan N and Bhat, Kumar MR and Rao, Mallikarjuna C and Nayak, Soubhagya Ranjan (2008) Petroleum ether extract of Cissus quadrangularis (LINN) stimulates the growth of fetal bone during intra uterine developmen tal period: a morph ometric analysis. CLINICS, 63 (6). pp. 815-820.

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Abstract

Objective: The aim of the present study was to analyze the effect Cissus quadrangularis plant petroleum ether extract on the development of long bones during the intra-uterine developmental stage in rats. Methods: Pregnant rats (n=12) were randomly assigned into either a control group (n=6) or a Cissus quadrangularis treatment (n=6) group. Pregnant rats in the Cissus quadrangularis group were treated with Cissus quadrangularis petroleum ether extract at a dose of 500 mg/kg body weight from gestation day 9 until delivery. The animals in the control group received an equal volume of saline. Newborn pups were collected from both groups for alizarin red S - alcian blue staining to differentiate ossified and unossified cartilage. The ossified cartilage (bone) was morphometrically analyzed using Scion image software. Results: Morphometric analysis revealed that the percentage of the total length of ossified cartilage (bone) in pups born to treated dams was significantly higher (P<0.001- 0.0001) than that of the control group. Conclusion: The results of the present study suggest that maternal administration of Cissus quadrangularis petroleum ether extract during pregnancy can stimulate the development of fetal bone growth during the intra-uterine developmental period.

Item Type: Article
Uncontrolled Keywords: Estrogens; Phytoestrogens; Ossification; Alizarin red-Alcian blue
Subjects: Medicine > KMC Manipal > Anatomy
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 16 Jun 2011 10:26
Last Modified: 07 Nov 2016 08:57
URI: http://eprints.manipal.edu/id/eprint/291

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