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Effect of Antioxidants (Vitamin C) on tissue ceruloplasmin following renal Ischemia reperfusion in wistar rats

*, Vinodini NA and Tripathi , Y and *, Raghuveer C V and *, Ranade AV and Kamath, Asha and *, Sheila R Pai (2012) Effect of Antioxidants (Vitamin C) on tissue ceruloplasmin following renal Ischemia reperfusion in wistar rats. International Journal of Biomedical and Advance Research, 3 (1). pp. 36-39.

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Abstract

Oxidant injury has been implicated in the pathogenesis of inflammatory, metabolic and toxic insults in ischemic –reperfusion injury. Oxidant injury is initiated by free radicals and reactive oxygen molecules .However when the cells are exposed to oxidant stress several different antioxidant defense systems operate to prevent or limit the injury. Ceruloplasmin one among such antioxidants; a powerful free radical scavenger. Aim: To study the effect of vitamin C on tissue ceruloplasmin level following renal reperfusion. Materials and Methods: Wistar albino rats were divided into Group I, II & III the Gr. II the experimental groups) were subjected to ischemia for 60 minutes followed by 24 hrs of reperfusion. The Gr.III was pre- treated with vitamin C (20mg/kg.bw) for 30 days followed by 60 minute ischemia & 24hrs of reperfusion. After the experimental procedure was over; the kidneys were removed and homogenized. The homogenized tissue was used for biochemical estimation of lipid peroxidation & ceruloplasmin. Result: A significant in the levels of tissue lipid peroxidation (MDA) and increase in tissue ceruloplasmin level was observed in Group II compared to those in Group I (normal control) However, the pre- treated group (Group III) showed an increase in the levels of ceruloplasmin and a decrease in lipid peroxidation in comparison to group II. Conclusion: The results of the present study suggest that administration of vitamin C prior to renal ischemia reperfusion protect the renal tissue from the free radical induced reperfusion injury.

Item Type: Article
Uncontrolled Keywords: renal ischemia reperfusion; MDA; ceruloplasmin; Vitamin C
Subjects: Medicine > KMC Manipal > Community Medicine
Medicine > KMC Mangalore > Physiology
Depositing User: KMCMLR User
Date Deposited: 06 Feb 2012 10:37
Last Modified: 06 Feb 2012 10:37
URI: http://eprints.manipal.edu/id/eprint/2985

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