Venkatesan, V and Chalsani, M and Nawaz, SS and Bhonde, RR and Challa, SS and Nappanveettil, G (2011) Optimization of condition(s) towards establishment of primary islet cell cultures from WNIN/Ob mutant rat. Cytotechnology. ISSN 1573-0778.

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Abstract

WNIN/Ob, a mutant rat strain, developed at the National Center for Laboratory Animal Sciences (NCLAS) facility of National Institute of Nutrition (NIN), is a new animal model to study the metabolic syndrome. These animals have 47% fat in their body and isolation of islets from these animals were compounded due to the formation of amorphous viscous and jelly like material which reduced the islet yield. However, islets isolated from WNIN adult (≥12 months) control rats gave a good islet recovery, under standard isolation procedures using collagenase digestion. In the present study we optimized culture conditions in WNIN/Ob rats to isolate islets with higher yield, and also established primary islet cell cultures from these mutant rats, retaining cellular integrity and functionality.

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