Optimization of condition(s) towards establishment of primary islet cell cultures from WNIN/Ob mutant rat.

Venkatesan, V and Chalsani, M and Nawaz, SS and Bhonde, RR and Challa, SS and Nappanveettil, G (2011) Optimization of condition(s) towards establishment of primary islet cell cultures from WNIN/Ob mutant rat. Cytotechnology. ISSN 1573-0778.

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Abstract

WNIN/Ob, a mutant rat strain, developed at the National Center for Laboratory Animal Sciences (NCLAS) facility of National Institute of Nutrition (NIN), is a new animal model to study the metabolic syndrome. These animals have 47% fat in their body and isolation of islets from these animals were compounded due to the formation of amorphous viscous and jelly like material which reduced the islet yield. However, islets isolated from WNIN adult (≥12 months) control rats gave a good islet recovery, under standard isolation procedures using collagenase digestion. In the present study we optimized culture conditions in WNIN/Ob rats to isolate islets with higher yield, and also established primary islet cell cultures from these mutant rats, retaining cellular integrity and functionality.

Item Type: Article
Additional Information: Copyright of this article is belongs to Springer.
Uncontrolled Keywords: Obese rats;Collagenase;Islet isolation;Islet cell culture;Viability and islet function
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 23 Feb 2012 08:21
Last Modified: 23 Feb 2012 08:21
URI: http://eprints.manipal.edu/id/eprint/3136

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