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Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective

Prabhakar, KR and Veeresh, VP and Vipan, K and Sudheer, M and Priyadarsini, KI and Satish, RBSS and Unnikrishnan, MK (2006) Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective. Phytomedicine, 13. pp. 591-595.

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Abstract

The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTSd�, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 mM DPPH at a concentration of 150 mg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 mM ABTSd� at a concentration of 300 mg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction.

Item Type: Article
Uncontrolled Keywords: Coronopus didymus, Free radical scavenging, Stop-flow spectrometry, Pulse radiolysis, Competition kinetics
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 15 Jul 2011 06:49
Last Modified: 15 Jul 2011 06:49
URI: http://eprints.manipal.edu/id/eprint/320

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