Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective

Prabhakar, KR and Veeresh, VP and Vipan, K and Sudheer, M and Priyadarsini, KI and Satish, RBSS and Unnikrishnan, MK (2006) Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective. Phytomedicine, 13. pp. 591-595.

PDF 617&ie=_sdarticle.pdf - Published Version Restricted to Registered users only Download (200kB) | Request a copy

Abstract

The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTSd�, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 mM DPPH at a concentration of 150 mg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 mM ABTSd� at a concentration of 300 mg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction.

Actions (login required)

View Item