Hepatoprotective activity of aloe vera gel against paracetamol induced hepatotoxicity in albino rats

Nayak, Veena and Gincy, TB and Prakash, M and Joshi, Joshi and Rao, Soumya S and Somayaji, SN and Nelluri, Venumadhav and Bairy, KL (2011) Hepatoprotective activity of aloe vera gel against paracetamol induced hepatotoxicity in albino rats. Asian Journal of Pharmaceutical and Biological Research, 1 (2). pp. 94-98.

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Abstract

Objectives: To study the hepatoprotective activity of aqueous extract of Aloe vera in paracetamol induced hepatotoxicity inalbino rats.Methods: Hepatotoxicity was induced with a single dose of paracetamol 3g/kg orally. The curative and regenerative effect ofAloe vera on paracetamol induced hepatotoxicitywas studied. N acetyl cysteine was used as standard treatment forhepatotoxicity in this study.The animals were divided into eight groups(n=6). First four groups received either vehicle (2% gumacacia),Aloe vera (250 mg/kg or 500 mg/kg ) or N- acetylcysteine ( 450mg/kg) single dose orally with paracetamol3g/kg orally.The next four groups of animals received the same treatment for 7 days along with single dose of paracetamol 3g/kg orally.After the treatment period the blood samples were collected from orbital sinus and aspartate transaminase (AST), alaninetransaminase (ALT) and alkaline phosphatase (ALP) were estimated.Liver homogenates were used for estimation of tissue thiollevels. Results: Paracetamol in the dose of 3g/kg significantly elevated AST , ALT and ALP and depleted the liver thiol levels whencompared with vehicle treated group. Single day treatment with the aqueous extract of Aloe vera in the dose of 250 and500mg/kg reduced the AST, and ALT levels significantly (p<0.01). 500mg /kg of the extract also reduced the ALP levels andrestored the depleted liver thiol levels significantly (p<0.01), however there was no effect on alkaline phosphatase and liverthiols by Aloe vera in the dose of 250mg/kg.Seven day treatment with both the doses of Aloe vera significantly reduced thelevels of AST, ALT and ALP significantly (p<0.01) and restored the depleted liver thiol levels significantly (p<0.01). Surprisingly, N-acetylcysteine the drug used to treat paracetamol toxicity on single or seven day treatment reduced only AST and ALT levelswith no effect on ALP and liver thiols. Conclusion: This study shows that the aqueous extract of Aloe vera has hepatoprotective activity

Item Type: Article
Uncontrolled Keywords: Aloe vera, hepatoprotective activity, paracetamol, liver function tests
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > KMC Manipal > Biochemistry
Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 23 Feb 2012 05:39
Last Modified: 24 Apr 2014 10:17
URI: http://eprints.manipal.edu/id/eprint/3241

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