High Performance Liquid Chromatographic Method for Determination of Clobetasol in Rat Plasma and its Application to Skin Penetration

Musmade, Prashant B and Deshpande, Praful B and Dengle, Swapnil J and Talole, Kranti B and Pathak, Shriram M and Adiga, MNS and Bhat, Krishnamurthy and Udupa, Nayanabhirama and Bairy, Laxminarayana K (2010) High Performance Liquid Chromatographic Method for Determination of Clobetasol in Rat Plasma and its Application to Skin Penetration. J Bioanal Biomed, 2 (1). pp. 1-7. ISSN 1948-593X JBABM

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Abstract

A simple and sensitive high performance liquid chromatography (HPLC) method was developed for quantification of clobetasol (CLB) in rat plasma. Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C18 (250.0 × 4.6 mm, 5 μm particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was validated as per the United States Food and Drug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32±1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.

Item Type: Article
Uncontrolled Keywords: Clobetasol, HPLC, Skin penetration, Zinc salt
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Medicine > KMC Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 20 Jun 2011 06:08
Last Modified: 12 Jan 2012 06:22
URI: http://eprints.manipal.edu/id/eprint/327

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