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Evaluation of the cytotoxic effect of the monoterpene indole alkaloid echitamine in-vitro and in tumour-bearing mice

Jagetia, Ganesh Chandra and Baliga, Manjeshwar Shrinath and Ponemone , Venkatesh and Ulloor, Jagadish N and Mantena, Sudheer Kumar and Genebriera, Joseph and Mathuram, V (2005) Evaluation of the cytotoxic effect of the monoterpene indole alkaloid echitamine in-vitro and in tumour-bearing mice. JPP, 57. pp. 1213-1219. ISSN 0022-3573

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Abstract

The cytotoxic effect of various concentrations of echitamine chloride was studied in HeLa, HepG2, HL60, KB and MCF-7 cell lines in-vitro and in mice bearing Ehrlich ascites carcinoma (EAC). Exposure of various cells to different concentrations of echitamine chloride resulted in a concentration-dependent cell killing, and KB cells were found to be most sensitive amongst all the cells evaluated. EAC mice treated with 1, 2, 4, 6, 8, 12 or 16mgkg�1 echitamine chloride showed a dose-dependent elevation in the anti-tumour activity, as evident by increased number of survivors in comparison with the non-drug treated controls. The highest dose of echitamine chloride (16mgkg�1) caused toxicity in the recipient mice, therefore 12mgkg�1 was considered the best cytotoxic dose for its anti-tumour effect. Administration of 12mgkg�1 echitamine chloride resulted in an increase in the median survival time (MST) up to 30.5 days, which was 11.5 days higher than the non-drug treated control (19 days). Administration of 16mgkg�1 echitamine chloride to EAC mice resulted in a time dependent elevation in lipid peroxidation that reached a peak at 6 h post-treatment, whereas glutathione concentration declined in a time dependent manner and a maximum decline was reported at 3 h post-treatment. Our study demonstrated that echitamine chloride possessed anti-tumour activity in-vitro and in-vivo.

Item Type: Article
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 15 Jul 2011 06:50
Last Modified: 15 Jul 2011 06:50
URI: http://eprints.manipal.edu/id/eprint/329

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