In vitro Free Radical Scavenging Potential of Defatted Ethanolic Extract of the Seeds of Lepidium sativum Linn

Shirwaikar, Annie and Patel, Bharatkumar and Kamariya, Yogesh and Parmar, Vinit and Khan, Saleemulla (2011) In vitro Free Radical Scavenging Potential of Defatted Ethanolic Extract of the Seeds of Lepidium sativum Linn. Chinese Journal of Natural Medicines, 9 (6). pp. 435-440.

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Abstract

AIM: The objective of the present study was to evaluate the antioxidant potential of the defatted ethanolic extract of the seeds of Lepidium Sativum Linn. METHODS: Different in vitro chemical assays viz. DPPH (1, 1-diphenyl-2 picrylhydrazyl) radical scavenging, ABTS (2, 2- azinobis- (3-ethylbenzothiazoline-6-sulphonate) radical scavenging, iron chelation, lipid peroxidation, superoxide scavenging and non-enzymatic haemoglobin glycosylation assay were used. The total antioxidant capacity of the extract was determined spectrophotometrically by phosphomolybdic acid method. RESULT & CONCLUSION: The defatted lepidium seed extract showed significant free radical scavenging activity in ABTS and non-enzymatic glycosylation assays, and a moderate activity in all the other assays. IC50 of the extract in the DPPH, ABTS, iron chelation, lipid peroxidation and super oxide scavenging assays were found to be 171.13, 38.64, 128.94, 71.39 and 206.09 μg·mL−1 respectively. The haemoglobin glycosylation assay of the extract showed a percentage scavenging of 46.60% and 74.88%, at 0.5 and 1.0 μg·mL−1, concentrations, respectively. Total antioxidant capacity of ethanolic extract of L. sativum (10 mg·mL−1) was found to be equivalent to 58.38 μg·mL−1 of ascorbic acid.

Item Type: Article
Uncontrolled Keywords: DPPH; ABTS; Lipid peroxidation; Nitric oxide; Lepidium sativum
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Depositing User: KMC Manipal
Date Deposited: 16 Mar 2012 06:42
Last Modified: 16 Mar 2012 06:42
URI: http://eprints.manipal.edu/id/eprint/3441

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