A Sweet Potion to Put Embryonic Stem Cells to Sleep

Deb, Kaushik D (2009) A Sweet Potion to Put Embryonic Stem Cells to Sleep. TheScientificWorldJOURNAL, 9. pp. 236-249. ISSN 1537-744X.

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Human embryonic stem cells (hESCs) are rapidly revolutionizing the areas of drug screening and therapy. In view of their applications and high operational costs at global multicentric setups, the ability to store and transport hESCs and derivatives under ambient temperatures, and their cryopreservation without compromising the stemness, function, and viability, is becoming imperative. Here we discuss the need for a natural cryoprotectant and biopreservative with a potential to improve cryopreservation, ambient temperature storage, and shipping of hESCs and derivatives. Trehalose, a naturally occurring disaccharide with therapeutic properties, protects the integrity of cells against desiccation, dehydration, and extreme heat or cold, and has been successfully tested for some somatic stem cell types. However, the biggest setback is the inability of mammalian cells to internalize trehalose. Here we review the methods being developed at different laboratories to facilitate its intercellular transport and advocate the need for similar advances in hESCs.

Item Type: Article
Additional Information: Copyright act of this article is belongs to The Scientific World.
Uncontrolled Keywords: trehalose;cryopreservation;off the shelf;toxicity epigenetics;storage;transportation;embryonic stem cells; embryos;natural cryoprotectants;biopreservatives;banking; therapy;drug screening.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 20 Mar 2012 06:48
Last Modified: 20 Mar 2012 06:48
URI: http://eprints.manipal.edu/id/eprint/3532

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