Propensity of Human Embryonic Stem Cell Lines During Early Stage of Lineage Specification Controls Their Terminal Differentiation into Mature Cell Types

Pal, Rajarshi and Totey, Swapnil and Mamidi, Murali Krishna and Bhat, Vijay S and Totey, Satish (2009) Propensity of Human Embryonic Stem Cell Lines During Early Stage of Lineage Specification Controls Their Terminal Differentiation into Mature Cell Types. Experimental Biology and Medicine, 234 (10). pp. 1230-1243. ISSN 1535-3699

[img] PDF
Propensity_of_Human_Embryonic.pdf - Published Version
Restricted to Registered users only

Download (2MB) | Request a copy
Official URL: http://ebm.rsmjournals.com/content/234/10/1230.sho...

Abstract

Human embryonic stem cells (hESCs) are able to stably maintain their characteristics for an unlimited period; nevertheless, substantial differences among cell lines in gene and protein expression not manifested during the undifferentiated state may appear when cells differentiate. It is widely accepted that developing an efficient protocol to control the differentiation of hESCs will enable us to produce adequate numbers of desired cell types with relative ease for diverse applications ranging from basic research to cell therapy and drug screening. Hence of late, there has been considerable interest in understanding whether and how hESC lines are equivalent or different to each other in their in vitro developmental tendencies. In this study, we compared the developmental competences of two hESC lines (HUES-9 and HUES-7) at molecular, cellular and functional levels, upon spontaneous differentiation without any added inducing agents. Both cell lines generated the three embryonic germ layers, extra-embryonic tissues and primordial germ cells during embryoid body (EB) formation. However HUES-9 showed a stronger propensity towards formation of neuroectodermal lineages, whereas HUES-7 differentiated preferentially into mesoderm and endoderm. Upon further differentiation, HUES-9 generated largely neural cells (neurons, oligodendrocytes, astrocytes and gangliosides) whereas HUES-7 formed mesendodermal derivatives, including cardiomyocytes, skeletal myocytes, endothelial cells, hepatocytes and pancreatic cells. Overall, our findings endorse the hypothesis that independently-derived hESCs biologically differ among themselves, thereby displaying varying differentiation propensity. These subtle differences not only highlight the importance of screening and deriving lines for lineage-specific differentiation but also indicate that individual lines may possess a repertoire of capabilities that is unique.

Item Type: Article
Additional Information: Copyright of this article is belongs to Royal Society of Medicine Press.
Uncontrolled Keywords: human embryonic stem cell lines;pluripotency;spontaneous differentiation;embryoid body;neuroectoderm;mesendoderm; propensity;characterization;marker expression.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 20 Mar 2012 06:49
Last Modified: 20 Mar 2012 06:49
URI: http://eprints.manipal.edu/id/eprint/3582

Actions (login required)

View Item View Item