In Vitro Cytotoxic Activity of Dendrophthoe falcata on Human Breast Adenocarcinoma cells-MCF-7

Dashora, Nipun and Sodde, Vijay and Prabhu, Kirti S and Lobo, Richard (2011) In Vitro Cytotoxic Activity of Dendrophthoe falcata on Human Breast Adenocarcinoma cells-MCF-7. International Journal of Cancer Research, 7 (1). pp. 47-54. ISSN 1811-9727

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Abstract

In vitro studies were carried out to evaluate the cytotoxic potential of the ethanolic and aqueous extracts from stem of Dendrophthoe falcata against human breast cancer cells (MCF-7). Screening of these extracts was done using the brine shrimp lethality bioassay, MTT assay and SRB assay. The results showed the significant decrease of the viability of the cells in a concentration-dependent manner. The ethanolic extract of D. falcata showed IC50 107 μg mL-1 in MTT assay and 112 μg mL-1 in SRB assay for MCF-7 cells and thus showed significant cytotoxic activity. The aqueous extract of D. falcata demonstrated higher activity with IC50 90 μg mL-1 in MTT and 98 μg mL-1 in SRB assay for MCF-7 cells, respectively after 48 h of exposure. In brine shrimp lethality bioassay also aqueous extract showed more potent cytotoxicity with LC50 90 μg mL-1 as compared to ethanolic extract with LC50 120 μg mL-1. From results obtained it was observed that both extracts of D. falcata may be a promising alternative to synthetic substances as natural compound with high antiproliferative activities and to be useful in cancer treatment and prevention.

Item Type: Article
Uncontrolled Keywords: Carcinogenesity,methanolic and aqueous extract, foetal bovine serum, Artemia salina
Subjects: Pharmacy > MCOPS Manipal > Pharmacognosy
Depositing User: KMC Manipal
Date Deposited: 19 Mar 2012 04:21
Last Modified: 19 Mar 2012 04:21
URI: http://eprints.manipal.edu/id/eprint/3594

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