Tippavajhala, Vamshi Krishna and Rao, Madhusudan Yamsani (2010) Electrophoretic Estimation of Insulin: A Novel Approach an Older Technique. International Journal of Current Pharmaceutical Research, 2 (4). pp. 19-23. ISSN 0975-1491

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Abstract

The objective of the study was to develop a simple and cost effective method for qualitative and quantitative determination of Insulin. In the present study, Insulin was quantitatively estimated Polyacrylamide Gel Electrophoresis (PAGE) and Gel Documentation. PAGE was used as the qualitative tool to develop the bands of insulin. The developed bands were quantified by Gel documentation system. Acrylamide was used as the gelling agent, bis‐acrylamide as the cross linking agent, Tris hydrochloride as the separating gel buffer and Coomassie brilliant blue was used as the staining solution in PAGE. A chitosan gel preparation containing 4IU of Insulin per 100μl was optimized and the stability studies for that gel preparation were conducted for 30days in refrigerated temperature using PAGE and Gel Documentation by comparing it with a marketed product. The stability subjected samples in refrigerated condition passed the stability test and the levels of Insulin were in the acceptable limits.

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