MU Digital Repository
Logo

In vitro evaluation of the antimicrobial efficacy of 5% Doxycycline, 0.2% Chlorhexidine gluconate and 2.5% Sodium hypochlorite used alone or in combinations against Enterococcus faecalis

Kini , Sandya. K and *, Kundabala M and bairy, Indira and Ballal , Vasudev and Shenoy, Ramya In vitro evaluation of the antimicrobial efficacy of 5% Doxycycline, 0.2% Chlorhexidine gluconate and 2.5% Sodium hypochlorite used alone or in combinations against Enterococcus faecalis. Endodontology.

[img]
Preview
PDF
1.pdf

Download (620kB)
[img]
Preview
PDF
2.pdf

Download (685kB)
[img]
Preview
PDF
3.pdf

Download (740kB)
[img]
Preview
PDF
4.pdf

Download (750kB)
[img]
Preview
PDF
5.pdf

Download (584kB)
[img]
Preview
PDF
6.pdf

Download (645kB)
[img]
Preview
PDF
7.pdf - Published Version

Download (649kB)

Abstract

The present in vitro study was undertaken to evaluate the antimicrobial efficacy of Solo doxycycline, 0.Zoo chlorhexidine gluconate and 2. Soto sodium hypochlorite when used alone or in combinations against Enterococcus faecalis. Agar diffusion test was done to evaluate the antimicrobial efficacy of the various intracanal irrigants, Enterococcus faecalis (ATCC 29212) was used as a test organism. The inoculum of E. faecalis was streaked on the agar plates. Using a punch, wells each measuring 6mm in diameter and 4mm depth were made and 50 ml of the experimental irrigant was pipetted into each well. A total of 200 wells were made, 40 for each group and the plates were placed in a carbon dioxide incubator at 370 C for 48 hours. The zones of inhibition were measured using a mm scale and the results statistically analyzed using ANOVA test and Tukeys HSD. The zones of inhibition recorded were 1 9. 90, 1 4.6 0, 24.75, 25. 58, 26 .86 mm for Gr oup A (chlorhexidine gluconate), Gr oup B (sodium hypochlorite), Group C (doxycycline), Group D (combination of doxycycline and sodium hypochlorite) and Group E (combination of doxycycline and chlorhexidine gluconate) respectively. Within the limitations of this experiment, the results of the present study concluded that a combination of Solo doxycycline and 0.2% chlorhexidine is the choice of irrigant against E.faecalis.

Item Type: Article
Subjects: Dentistry > MCODS Mangalore > Public Health Dentistry
Depositing User: KMCMLR User
Date Deposited: 16 Apr 2012 05:12
Last Modified: 22 Apr 2014 10:07
URI: http://eprints.manipal.edu/id/eprint/3718

Actions (login required)

View Item View Item