Petroleum ether extract of cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis

Potu, Bhagath Kumar and Bhat, Kumar MR and Rao, Muddanna S and Nampurath, Gopalan Kutty and Chamallamudi, Mallikarjuna Rao and Nayak, Soubhagya Ranjan and Muttigi, Manjunatha S (2009) Petroleum ether extract of cissus quadrangularis (Linn.) enhances bone marrow mesenchymal stem cell proliferation and facilitates osteoblastogenesis. Clinics, 64 (10). pp. 993-998.

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Abstract

Objective: To evaluate the effects of the petroleum ether extract of Cissus quadrangularis on the proliferation rate of bone marrow mesenchymal stem cells, the differentiation of marrow mesenchymal stem cells into osteoblasts (osteoblastogenesis) and extracellular matrix calcification. This study also aimed to determine the additive effect of osteogenic media and Cissus quadrangularis on proliferation, differentiation and calcification. Methods: MSCs were cultured in media with or without Cissus quadrangularis for 4 weeks and were then stained for alkaline phosphatase. Extracellular matrix calcification was confirmed by Von Kossa staining. marrow mesenchymal stem cells cultures in control media and osteogenic media supplemented with Cissus quadrangularis extract (100, 200, 300 μg/mL) were also subjected to a cell proliferation assay (MTT). Results: Treatment with 100, 200 or 300 μg/mL petroleum ether extract of Cissus quadrangularis enhanced the differentiation of marrow mesenchymal stem cells into ALP-positive osteoblasts and increased extracellular matrix calcification. Treatment with 300 μg/mL petroleum ether extract of Cissus quadrangularis also enhanced the proliferation rate of the marrow mesenchymal stem cells. Cells grown in osteogenic media containing Cissus quadrangularis exhibited higher proliferation, differentiation and calcification rates than did control cells. Conclusion: The results suggest that Cissus quadrangularis stimulates osteoblastogenesis and can be used as preventive/alternative natural medicine for bone diseases such as osteoporosis.

Item Type: Article
Uncontrolled Keywords: Cissus quadrangularis, Mesenchymal stem cells, Osteoblastogenesis, Osteoporosis, Osteoblast
Subjects: Medicine > KMC Manipal > Anatomy
Medicine > KMC Manipal > Biochemistry
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 28 Jun 2011 06:16
Last Modified: 28 Jun 2011 06:16
URI: http://eprints.manipal.edu/id/eprint/411

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