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Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow

Sudheer Kumar, M and Unnikrishnan, MK and Uma Devi, P (2003) Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow. Mutation Research, 527. pp. 7-14.

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Abstract

5-Aminosalicylic acid (5ASA), a prescribed drug for ulcerative colitis, is a potent scavenger of oxygen-derived free radicals. The present studywas undertaken to ascertain its ability to protect against radiation-induced damage. The drug dose-dependent effect, optimum time of drug administration and radiation dose-dependent effect (0–4 Gy) on in vivo radiation protection against micronuclei induction in polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) were studied in the bone marrow of mice. Intraperitoneal injection of 10–125 mg/kg of the drug 30 min before whole body irradiation with 3Gy produced a significant reduction in the frequency of micronucleated erythrocytes at 24 h after exposure. The optimum dose for protection without drug toxicity was 25mg/kg body weight. Injection of 25 mg/kg of the drug 60 or 30 min before or within 15 min after 3Gy whole body �-irradiation resulted in a significant decrease in the radiation-induced PCE and NCE with micronuclei (MPCE and MNCE) and an increase in the ratio of PCE to NCE (P/N), at 24 h post-irradiation. Maximum effect was seen when the drug was administered 30 min before irradiation. Therefore, to study the radiation dose–response, mice were pre-treated with 25 mg/kg of 5ASA 30 min before 1–4 Gy of �-irradiation. Radiation increased the MN frequency linearly (r2 = 0.99) with dose. Pre-treatment with 5ASA significantly reduced the MN counts to 40–50% of the radiation (RT) alone values, giving a dose modification factor (DMF) of 2.02 (MPCE) and 2.53 (MNCE). Irradiation resulted in a dose-dependent decline in the P/N ratio at all the doses of radiation studied. 5ASA produced a significant increase in the P/N ratio from that of irradiated controls, at all doses of radiations tested. These results show that 5ASA protect mice against radiation-induced MN formation and mitotic arrest.

Item Type: Article
Uncontrolled Keywords: 5-Aminosalicylic acid (5ASA), Radioprotection, Micronuclei, Polychromatic erythrocytes, Normochromatic erythrocytes, Mouse bone marrow
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 13 Jul 2011 06:19
Last Modified: 13 Jul 2011 06:20
URI: http://eprints.manipal.edu/id/eprint/517

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