Surulivelrajan, Mallayasamy and Arumugam, Karthik and Jain, Tarun and Rajakannan, Thiyagu and Bhat, Krishnamurthy and Rao, Padma Gurumadhav and Devarakonda, Ramakrishna (2010) A Sensitive and Selective HPLC Method for Estimation of Lamotrigine in Human Plasma And Saliva: Application To Plasma-Saliva Correlation In Epileptic Patients. Arzneimittelforschung, 60 (10). pp. 599-606.

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Abstract

A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for estimation oflamotrigine (CAS 84057-84-1) in human plasma and saliva. The chromatographic separation was achieved with a reversed phase column and a mobile phase consisting of acetonitrile and 20 mM ammonium acetate buffer pH 6.5 (30: 70) with a flow rate of 1 mLimin. The calibration curve was linear within the working range for both plasma and saliva. The validated method has been successfully applied for a study of lamotrigine in human plasma and saliva to establish the correlation between these two matrices. A scatter plot of plasma versus salivary lamotrigine concentrations showed a good linear relationship them. (Pearson correlation cokinetics, plasma-saliva efficient, r = 0.6832, P < 0.001).

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