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Fluorimetric determination of rivastigmine in rat plasma by a reverse phase - High performance liquid chromatographic method

Arumugam, Karthik and Sundarajan, Ganesa Subramanian and Surulivelrajan, Mallayasamy and Averineni, Ranjith Kumar and Kamat, Suresh B (2008) Fluorimetric determination of rivastigmine in rat plasma by a reverse phase - High performance liquid chromatographic method. Arzneimittel-Forschung/Drug Research, 58 (5). pp. 205-210.

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Abstract

A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for the rapid quantification of rivastigmine (CAS 123441-03-2) in micro quantity in rat plasma samples. The chromatographic separation was achieved with a reverse phase monomeric column C18 (4.6 x 250 mm, 5 μm) and the mobile phase consisted of acetonitrile and 20 mmol/L phosphate buffer pH 3.0 (25:75) with a flow rate of 1 mL/min. The effluents were measured by fluorimetric detection with excitation and emission wavelengths at 220 nm and 293 nm, respectively. The calibration curve was linear (r2 >0.99) ranging from 25-3000 ng/mL and the lower limit of quantification was 25 ng/mL. The method was validated with excellent sensitivity, selectivity, accuracy, precision, recovery and stability. The method has been successfully applied in a pharmacokinetic study of rivastigmine in rats. © ECV Editio Cantor Verlag.

Item Type: Article
Uncontrolled Keywords: Acetylcholine esterase inhibitors, CAS 123441-03-2, Rivastigmine, bioanalytical method, fluorimetric detection, HPLC, rat plasma, validation
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Manipal
Date Deposited: 11 Jul 2011 05:11
Last Modified: 02 Nov 2016 09:28
URI: http://eprints.manipal.edu/id/eprint/646

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