Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector

Veerapur, VP and Prabhakar, KR and Parihar, Vipan kumar and Reddy Kandadi, Machendar and Ramakrishana, S and Mishra, B and Satish Rao, BS and Srinivasan, KK and Priyadarsini, KI and Unnikrishnan, MK (2009) Ficus racemosa Stem Bark Extract: A Potent Antioxidant and a Probable Natural Radioprotector. Evid Based Complement Alternat Med., 6 (3). pp. 317-324.

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Ethanol extract (FRE) and water extract (FRW) of Ficus racemosa (family: Moraceae) were subjected to free radical scavenging both by steady state and time resolved methods such as nanosecond pulse radiolysis and stopped-flow spectrophotometric analyses. FRE exhibited significantly higher steady state antioxidant activity than FRW. FRE exhibited concentration dependent DPPH, ABTS��, hydroxyl radical and superoxide radical scavenging and inhibition of lipid peroxidation with IC50 comparable with tested standard compounds. In vitro radioprotective potential of FRE was studied using micronucleus assay in irradiated Chinese hamster lung fibroblast cells (V79). Pretreatment with different doses of FRE 1h prior to 2 Gy g-radiation resulted in a significant (P<0.001) decrease in the percentage of micronucleated binuclear V79 cells. Maximum radioprotection was observed at 20 mg/ml of FRE. The radioprotection was found to be significant (P<0.01) when cells were treated with optimum dose of FRE (20 mg/ml) 1 h prior to 0.5, 1, 2, 3 and 4 Gy g-irradiation compared to the respective radiation controls. The cytokinesis-block proliferative index indicated that FRE does not alter radiation induced cell cycle delay. Based on all these results we conclude that the ethanol extract of F. racemosa acts as a potent antioxidant and a probable radioprotector.

Item Type: Article
Uncontrolled Keywords: Chinese hamster lung fibroblast (V79) cells, Ficus racemosa, free radical scavenger, micronucleus assay, radioprotection
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Chemistry
Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 11 Jul 2011 06:05
Last Modified: 11 Jul 2011 06:05

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