Chromium (D-Phenylalanine)3 alleviates high fat-induced insulin resistance and lipid abnormalities

Machender Reddy, Kandadi and Unnikrishnan, MK and Warrier, Ajaya Kumar Sankara and Min, Du and Jun, Ren and Nair, Sreejayan (2011) Chromium (D-Phenylalanine)3 alleviates high fat-induced insulin resistance and lipid abnormalities. Journal of Inorganic Biochemistry, 105. pp. 58-62.

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Abstract

High-fat diet has been implicated as a major cause of insulin resistance and dyslipidemia. The objective of this study was to evaluate the impact of dietary-supplementation of chromium (D-phenylalanine)3 [Cr(D-Phe)3] on glucose and insulin tolerance in high-fat diet fed mice. C57BL/6-mice were randomly assigned to orally receive vehicle or Cr(D-Phe)3 (45 μg of elemental chromium/kg/day) for 8-weeks. High-fat-fed mice exhibited impaired whole-body-glucose and -insulin tolerance and elevated serumtriglyceride levels compared to normal chow-fed mice. Insulin-stimulated glucose up-take in the gastrocnemius muscles, assessed as 2-[3H-deoxyglucose] incorporation was markedly diminished in high-fat fed mice compared to control mice. Treatment with chromium reconciled the high-fat diet-induced alterations in carbohydrate and lipid metabolism. Treatment of cultured, differentiated myotubes with palmitic acid evoked insulin resistance as evidenced by lower levels of insulin-stimulated Akt-phosphorylation, elevated JNK-phosphorylation, (assessed by Western blotting), attenuation of phosphoinositol-3-kinase activity (determined in the insulin-receptor substrate-1-immunoprecipitates by measuring the extent of phosphorylation of phosphatidylinositol by γ-32P-ATP), and impairment in cellular glucose up-take, all of which were inhibited by Cr(D-Phe)3. These results suggest a beneficial effect of chromium-supplementation in insulin resistant conditions. It is likely that these effects of chromium may be mediated by augmenting downstream insulin signaling.

Item Type: Article
Uncontrolled Keywords: Chromium High-fat diet Insulin resistance
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 19 Jul 2011 04:39
Last Modified: 19 Jul 2011 04:41
URI: http://eprints.manipal.edu/id/eprint/718

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