Rapid Quantification of Irinotecan In Rat Plasma By Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry-Application To Preclinical Pharmacokinetics

Shetty, R and Kumar, V and Gajjar, AK and Patel, J and Reddy, CM and Theerthahalli, Arun Sudheendra and Bairy, KL (2009) Rapid Quantification of Irinotecan In Rat Plasma By Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry-Application To Preclinical Pharmacokinetics. Pharmacologyonline, 2. pp. 605-615.

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Abstract

A simple rapid and robust method for quantification of irinotecan in rat plasma has been established using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Imipramine was used as an internal standard. Irinotecan and internal standard in plasma sample was extracted using simple liquid-liquid extraction. The samples were injected in to a C18 reversed-phase column and mobile phase used was acetonitrile – 0.1% formic acid in water (90:10, %v/v) at a flow rate of 0.5 ml/min. using MS/MS in the selected reaction-monitoring (SRM) mode, Irinotecan and Imipramine were detected without any interference from rat plasma matrix. Irinotecan roduced a precursor ion ([M+H]+) at m/z 587.20 and a corresponding product ion at m/z 167.01 The internal standard produced a precursor ion ([M+H]+) at m/z 281.20 and a corresponding product ion at m/z 86.19. Detection of Irinotecan in rat plasma by the LC-ESI/MS/MS method was accurate and precise with a quantitation limit of 10ng/ml. The proposed method was validated with linear range of 10 - 4000 ng/ml. Reproducibility, recovery and stability of the method were evaluated. The method has been successfully applied to preclinical pharmacokinetic study of Irinotecan liposome

Item Type: Article
Uncontrolled Keywords: Irinotecan, liquid chromatography, mass spectrometry, rat plasma.
Subjects: Pharmacy > MCOPS Manipal > Pharmacology
Depositing User: KMC Manipal
Date Deposited: 13 Aug 2011 07:20
Last Modified: 18 Oct 2013 09:41
URI: http://eprints.manipal.edu/id/eprint/743

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