Molecular analysis of oral squamous cell carcinoma: A tissue microarray study

Solomon, Monica C and Carnelio, S and Guddattu, Vasudeva (2010) Molecular analysis of oral squamous cell carcinoma: A tissue microarray study. Indian journal of cancer, 47 (2). pp. 166-172.

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Abstract

Background : An intriguing aspect of Oral Squamous Cell Carcinomas (OSCC) is its behavioral disparity. Among patients who present with the similar clinicopathological features, some have a better prognosis than others. Identification of molecular alterations responsible for this may contribute to a greater understanding of tumor behavior. Tissue microarray (TMA) approach is a high throughput technology that enables analysis of multiple molecular targets simultaneously without causing any morphological alteration to tissue specimens. Aim and Objective : To assess the tumor behavior based on the expression of p53, Bcl-2 and E-cadherin using TMA technology. Settings and Design : This was a case series analysis using tissue microarray technology. Materials and Methods : Formalin-fixed Paraffin-embedded (FFPE) tissue blocks of histological proven cases of OSCC (n = 30) were retrieved from the department archives. Tissue microarray blocks were constructed; 4 ΅m thick sections were cut and immunostained for p53, Bcl-2 and E-cadherin. Stastistical Analysis : Mean (SD) was used to summarize age, frequencies with percentages was used to summarize categorical variable and Chi-square test was used to find association between histopathology evaluation and expression of Bcl-2, p53, E-cadherin. Results and Conclusion : Bcl-2 was the most frequently expressed biomarker. The expression of Bcl-2 was inversely related to the degree of differentiation (P = 0.005). The follow-up data showed that 63.6% of the cases that were positive for both Bcl-2 and E-cadherin were disease-free following treatment. Tissue microarray technology is a promising way to analyse multiple biomarkers simultaneously. The molecular data obtained from TMA will enhance diagnosis, provide better prognostication and will improve cancer treatment for individual patients.

Item Type: Article
Uncontrolled Keywords: Molecular screening, tissue microarray, oral squamous cell carcinoma
Subjects: Dentistry > MCODS Manipal > Oral Pathology and Microbiology
Departments at MU > Statistics
Depositing User: KMC Manipal
Date Deposited: 13 Aug 2011 06:16
Last Modified: 07 Nov 2016 16:05
URI: http://eprints.manipal.edu/id/eprint/750

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