A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma—Application to preclinical pharmacokinetics

Pathak, Shriram M and Kumar, Ranjith A and Musmade, Prashant B and Udupa, N (2008) A simple and rapid high performance liquid chromatographic method with fluorescence detection for the estimation of fexofenadine in rat plasma—Application to preclinical pharmacokinetics. Talanta, 76. pp. 338-346. ISSN 0039-9140

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Abstract

A sensitive high performance liquid chromatographic (HPLC) method involving fluorescence detection was developed for the determination of fexofenadine (FEX), known to have low oral bioavailability, in rat plasma. In order to understand the effect of various chromatographic factors on the separation of analytes and to simultaneously optimize the resolution and analysis run time, a response surfacemethodwas used. The chromatographic separationwas achieved using a Supelco C18-DB (250mm×4.6mmI.D./5�mparticle size) column with mobile phase comprising of ammonium acetate buffer and acetonitrile (63:37, v/v), delivered isocratically at a flow rate of 1.0mL min−1. Diphenhydramine was used as an internal standard (I.S.). The statistical evaluation of the method was examined and the method was found to be precise and accurate with a linearity range of 1–500 ngmL−1 (r > 0.9980). The intra- and inter-day precision studies showed good reproducibility with coefficients of variation (C.V.) less than 12.26%. The advantages of our method are small sample volume (100�L), short time of analysis (13 min) and a simple sample extraction and clean-up as compared to the previously published methods. The established method provides a reliable bioanalytical methodology to carry out FEX pharmacokinetics in rat plasma.

Item Type: Article
Uncontrolled Keywords: Fexofenadine ,Bioanalytical method validation, Rat plasma, Preclinical pharmacokinetics.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Manipal
Date Deposited: 21 Apr 2012 06:54
Last Modified: 05 Nov 2016 09:59
URI: http://eprints.manipal.edu/id/eprint/76283

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