Analytical method development and validation for aspirin

Kumar, Suresh S and Jamadar, Latif D and Bhat, Krishnamurthy and Musmade, Prashant B and Vasantharaju, SG and Udupa, N (2010) Analytical method development and validation for aspirin. International Journal of ChemTech Research, 2 (1). pp. 389-399. ISSN 0974-4290

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A sensitive, specific, precise and cost effective High Performance Liquid Chromatographic method of analysis for aspirin in presence of its degradation products is developed and validated. The method employed Hypersil BDS C18 (100 x 4.6 mm 5μ) column as stationary phase.The mobile phase consisted of sodium perchlorate buffer (pH 2.5): acetonitrile: isopropyl alcohol (85:14:1 % v/v). It is pumped through the chromatographic system at a flow rate of 1.5 ml min−1.The UV detector is operated at 275 nm. This system was found to give good resolution between aspirin and its degradation products. Method was validated as per ICH guidelines.

Item Type: Article
Uncontrolled Keywords: Aspirin, HPLC, UV detector.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Manipal
Date Deposited: 21 Apr 2012 07:21
Last Modified: 21 Apr 2012 07:21

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