Erythrocyte Glutathione-S-Transferase Activity in Diabetics and its Association with HBA1c

Velladath, Saleena U and Das, Asha and Kumar, Rishikesh and Nalini, K (2011) Erythrocyte Glutathione-S-Transferase Activity in Diabetics and its Association with HBA1c. Clinical Biochemistry, 2 (7). pp. 1-7.

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Abstract

The generation of reactive oxygen species is increased in both types of diabetes and is closely associated with oxidative stress. Erythrocytes are equipped with a highly effective anti-oxidant defence system. Oxidative denaturation of hemoglobin leads to the release of hemin into the RBC membrane and the released hemin is capable of oxidizing membrane proteins via a thiyl radical intermediate. GST can bind free hemin that is released during Hb oxidation presumably reducing damage to RBC membrane. We hypothesized that as oxidative stress and hyperglycemia are major etiologic and pathologic factors of diabetes mellitus, then the antioxidant enzyme, Glutathione – S – Transferase (GST) in red blood cells, are likely to have a role in the glycation of Hb in diabetic patients. Hence we designed this study to evaluate the activity of red cell Glutathione-S-Transferase and correlate its association with HbA1c. This is a cross – sectional study, conducted in subjects attending Kasturba Hospital, Manipal. All patients who were advised to do a blood test for glycated haemoglobin were included in this study. Hemolysed blood samples were excluded. Erythrocyte GST activity was determined using the method of Habig et al and glycated Hb (HbA1c) was estimated using the turbidimetric inhibition immunoassay (TINIA) for hemolyzed whole blood by Roche Cobas Integra 400 auto analyzer. 68 patients who were confirmed to have type 2 diabetes mellitus were included in diabetic group whereas rest 32 patients without diabetes were included in non-diabetic group. The data were analysed using Mann Whitney U test and Pearson correlation coefficient test by SPSS (v. 14.0) software. From this study we could found out that there is no significant difference in erythrocyte GST activity between diabetics and non-diabetics (p value = 0.08). However a positive correlation between erythrocyte GST activity and HbA1c concentration was observed in diabetic patients (r = 0.239, p = 0.089 ). The chronicity of the disease along with treatment modalities might have played a significant role in the outcome of our study as the subjects included in diabetic group were selected without considering their glycemic control status.

Item Type: Article
Uncontrolled Keywords: Glutathione-S-transferase, Glycated Hemoglobin (HbA1c), Glutathione, Antioxidant Enzymes, Free Radicals, Oxidative Stress
Subjects: Allied Health > MCOAHS Manipal > Medical Laboratory Tech
Medicine > KMC Manipal > Biochemistry
Depositing User: KMC Manipal
Date Deposited: 09 May 2012 04:15
Last Modified: 09 May 2012 04:15
URI: http://eprints.manipal.edu/id/eprint/76386

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