Salian, Sujit Raj and Kalthur, Guruprasad and Uppangala, Shubhashree and Kumar, Pratap (2012) Frozen-thawed spermatozoa from oligozoospermic ejaculates are susceptible to in situ DNA fragmentation in polyvinylpyrrolidone-based sperm-immobilization medium. American Society for Reproductive Medicine. pp. 1-5.
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Abstract
To elucidate the effect of sperm immobilization media that are and are not based on polyvinylpyrrolidone (PVP) on the DNAintegrity of fresh and frozen-thawed spermatozoa during standard intracytoplasmic sperm injection (ICSI) conditions. Design: Experimental prospective study. Setting: Embryology research laboratory. Patient(s): Forty-six ejaculates from normozoospermic and oligozoospermic men. Intervention(s): Assessment of sperm DNA fragmentation by single-cell gel electrophoresis assay. Main Outcome Measure(s): DNA integrity of fresh and frozen-thawed spermatozoa from normozoospermic and oligozoospermic ejaculates exposed to PVP-based and non-PVP-based media. Result(s): Exposure of fresh and frozen thawed spermatozoa from normozoospermic and oligozoospermic ejaculates to PVP-based medium in an ICSI dish for 30 minutes statistically significantly increased the DNA fragmentation. In contrast, the extent of DNA fragmentation in non-PVP-based medium did not statistically significantly differ from control. Conclusion(s): A PVP-based medium can induce a statistically significant amount of sperm DNA fragmentation in an ICSI dish, and frozen-thawed sperm from oligozoospermic ejaculates are more susceptible to in situ DNA fragmentation.
Item Type: | Article |
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Uncontrolled Keywords: | Frozen thawed sperm, ICSI, oligozoospermia, PVP, sperm DNA damag |
Subjects: | Medicine > KMC Manipal > Clinical Embryology |
Depositing User: | KMC Manipal |
Date Deposited: | 23 May 2012 04:21 |
Last Modified: | 17 Aug 2021 09:57 |
URI: | http://eprints.manipal.edu/id/eprint/76489 |
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