Can Tissue PCR Augment the Diagnostic Accuracy in Genitourinary Tract Tuberculosis?

Chawla, Arun and Chawla, Kiran and Reddy, Sreedhar and Arora, Nitika and Bairy, Indira and Rao, Sugandhi and Hegde, Padmaraj and Thomas, Joseph (2012) Can Tissue PCR Augment the Diagnostic Accuracy in Genitourinary Tract Tuberculosis? Urologia internationalis, 88 (1). pp. 1-5. ISSN 0042–1138

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Abstract

Purpose: Conventional methods like smear and culture for Mycobacterium tuberculosis are of limited sensitivity and specificity. Histopathological examination (HPE) for the tissues obtained gives inconclusive diagnosis in the absence of caseous necrosis or stained acid-fast bacilli. This study was conducted to determine the utility of tissue PCR for diagnosing tuberculosis of the genitourinary tract (GUTB) and its comparative evaluation with HPE. Patients and Methods: A prospective study was conducted from January 2006 to August 2009 with 78 tissue specimens (renal, prostate, epididymis, penile and soft tissue) from patients with clinically suspected GUTB. All the samples were processed for both PCR and histopathology. Results: In 68 (87.1%) samples, results for both PCR and HPE were coinciding. False positivity and false negativity was observed in 5.1% (4/78) and 7.6% (6/78) samples, respectively. With HPE as the gold standard, PCR has shown sensitivity of 87.5% (95% CI 80.1; 91.9) and specificity of 86.7% (95% CI 74.9; 93.8) and positive agreement between two tests was observed as significant (0.7). PCR results were obtained within a mean period of 3.4 days while those of HPE were obtained in 7.2 days. Conclusions: Tissue PCR is a sensitive and specific method for obtaining early and timely diagnosis of GUTB. Application of tissue PCR results can augment the diagnostic accuracy in histopathologically labelled granulomatous inflammations.

Item Type: Article
Uncontrolled Keywords: Genitourinary tract , Tuberculosis , Tissue PCR , Histopathology
Subjects: Medicine > KMC Manipal > Microbiology
Medicine > KMC Manipal > Urology
Depositing User: KMC Manipal
Date Deposited: 31 May 2012 04:31
Last Modified: 31 May 2012 04:31
URI: http://eprints.manipal.edu/id/eprint/76552

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