Honey as an antimicrobial agent against Pseudomonas aeruginosa isolated from infected wounds

Shenoy, Vishnu Prasad and Ballal, Mamatha and Shivananda, PG and Bairy, Indira (2012) Honey as an antimicrobial agent against Pseudomonas aeruginosa isolated from infected wounds. Journal of Global Infectious Diseases, 4 (2). pp. 102-105. ISSN 0974-777X

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Abstract

Background: As natural products garner attention in the medical field due to emergence of antibiotic resistant strains of bacteria, honey is valued for its antibacterial activity. Objective: Fifty strains of Pseudomonas aeruginosa isolated from infected wounds were evaluated for their antibacterial action using honey in comparison with different antibiotics and Dettol. Methodology and Results: All the strains were found to be sensitive to honey at a minimum inhibitory concentration of 20% in comparison with Dettol at 10% using agar dilution method. In the second step, the time kill assay was performed on five isolates of P. aeruginosa to demonstrate the bactericidal activity of honey at different dilutions of honey ranging from 20% to 100% at regular time intervals. All the isolates of P. aeruginosa tested were killed in 12-24 h depending on the dilutions of the honey tested. Thus, honey could prevent the growth of P. aeruginosa even if it was diluted by deionized water by fivefolds in vitro. Honey had almost uniform bactericidal activity against P. aeruginosa irrespective of their susceptibility to different classes of antibiotics. Conclusion: Honey which is a natural, non-toxic, and an inexpensive product has activity against the P. aeruginosa isolated from infected wounds may make it an alternative topical choice in the treatment of wound infections.

Item Type: Article
Uncontrolled Keywords: Antibacterial activity, Honey, P. aeruginosa
Subjects: Medicine > MMMC Manipal > Microbiology
Medicine > KMC Manipal > Microbiology
Depositing User: KMC Manipal
Date Deposited: 08 Jun 2012 04:53
Last Modified: 08 Jun 2012 04:53
URI: http://eprints.manipal.edu/id/eprint/76610

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