Glycosaminoglycans enhance osteoblast differentiation of bone marrow derived human mesenchymal stem cells.

Mathews, Smitha and Mathew, Suja Ann and Gupta, Pawan Kumar and Bhonde, Ramesh R and Totey, Satish M (2012) Glycosaminoglycans enhance osteoblast differentiation of bone marrow derived human mesenchymal stem cells. Journal of Tissue Engineering and Regenerative Medicine.

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Abstract

Extracellular matrix plays an important role in regulating cell growth and differentiation. The biomimetic approach of cell-based tissue engineering is based on mirroring this in vivo micro environment for developing a functional tissue engineered construct. In this study, we treated normal tissue culture plates with selected extracellular matrix components consisting of glycosaminoglycans such as chondroitin-4-sulphate, dermatan sulphate, chondroitin-6-sulphate, heparin and hyaluronic acid. Mesenchymal stem cells isolated from adult human bone marrow were cultured on the glycosaminoglycan treated culture plates to evaluate their regulatory role in cell growth and osteoblast differentiation. Although no significant improvement on human mesenchymal stem cell adhesion and proliferation was observed on the glycosaminoglycan-treated tissue culture plates, there was selective osteoblast differentiation, indicating its potential role in differentiation rather than proliferation. Osteoblast differentiation studies showed high osteogenic potential for all tested glycosaminoglycans except chondroitin-4-sulphate. Osteoblast differentiation-associated genes such as osterix, osteocalcin, integrin inding sialoprotein, osteonectin and collagen, type 1, alpha 1 showed significant upregulation. We identified osterix as the key transcription factor responsible for the enhanced bone matrix deposition observed on hyaluronic acid, heparin and chondroitin-6-sulphate. Hyaluronic acid provided the most favourable condition for osteoblast differentiation and bone matrix synthesis. Our results confirm and emphasise the significant role of extracellular matrix in regulating cell differentiation. To summarise, glycosaminoglycans of extracellular matrix played a significant role in regulating osteoblast differentiation and could be exploited in the biomimetic approach of fabricating or functionalizing scaffolds for stem cell based bone tissue engineering.

Item Type: Article
Additional Information: Copyright of this article is belongs to John Wiley & Sons, Ltd.
Uncontrolled Keywords: extracellular matrix;mesenchymal stem cells;osteoblast differentiation;hyaluronic acid;glycosaminoglycans; osterix; bone tissue engineering; chondroitin sulphate.
Subjects: Regenerative Medicine > MIRM Bangalore
Depositing User: MCON Library
Date Deposited: 08 Jun 2012 09:21
Last Modified: 08 Jun 2012 09:29
URI: http://eprints.manipal.edu/id/eprint/76619

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