Development of a sensitive bioanalytical method for the quantification of lacosamide in rat plasma. Application to preclinical pharmacokinetics studies in rats.

Shah, SK and Vasantharaju, SG and Arumugam, K and Muddukrishna, BS and Desai, N (2012) Development of a sensitive bioanalytical method for the quantification of lacosamide in rat plasma. Application to preclinical pharmacokinetics studies in rats. Arzneimittelforschung, 62 (5). pp. 243-246. ISSN 0004-4172

[img] PDF
S._G._Vasantharaju_MAHE_00006493.pdf - Published Version
Restricted to Registered users only

Download (820kB) | Request a copy

Abstract

A sensitive and selective high performance liquid chromatographic (HPLC) method was developed and validated for quantifi cation of lacosamide in rat plasma. A liquid-liquid extraction procedure was optimized to extract lacosamide from rat plasma. Chromatographic separation was accomplished using a reversed phase C 18 Hichrom (250 × 4.6 mm, 5 μm) column with the mobile phase consisting of acetonitrile-phosphate buff er (pH 3.2 ± 0.1; 20 mM) (21:79, v/v) at a fl ow rate of 1 mL/min. Both intra- and inter day assay precision and accuracy were lower than 15 % CV. The lower limit of quantitation was 25 ng/mL for lacosamide and the response was linear in a concentration range from 25 to 10 000 ng/mL. The developed method was successfully used for the preclinical pharmacokinetic study of lacosamide in rats

Item Type: Article
Uncontrolled Keywords: lacosamide, HPLC method, rat plasma, pharmacokinetics, validation.
Subjects: Pharmacy > MCOPS Manipal > Pharmaceutical Quality Assurance
Depositing User: KMC Manipal
Date Deposited: 11 Jun 2012 05:10
Last Modified: 11 Jun 2012 05:10
URI: http://eprints.manipal.edu/id/eprint/76635

Actions (login required)

View Item View Item