Design,development and evaluation of fiber optic probe based He-Ne-low level laser therapy system for tissue regeneration

Prabhu, Vijendra and Rao, Satish BS and Rao, Nageshwara and Aithal, Kiran and Kumar, Pramod and Mahato, Krishna Kishore (2010) Design,development and evaluation of fiber optic probe based He-Ne-low level laser therapy system for tissue regeneration. Photochemistry and Photobiology, 86 (6). pp. 1364-1372.

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Abstract

Background: The use of phototherapy in the field of wound healing has gained popularity over the years. In phototherapy, asuitable irradiation is applied on the wounds, accelerates healing process. In the current study our aim was to design and develop a fiber optic probe based low level laser therapy (LLLT) system using Helium- Neon (He-Ne) laser and to optimize the laser dose and treatment schedule for tissue regeneration on Swiss albino mice. For selecting optimum laser dose, the animal wounds were exposed with different doses (1, 2, 3, 4, 6, 8 and 10 J/cm2) separately except controls. The standardization of treatment schedule was carried out by irradiating wounds to 2 J/cm2 at different post wounding time (0, 24 and 48 h). Study: Auxiliary components were designed and fabricated for the development of the low level laser therapy system. Tissue regeneration potential of the system was evaluated by following the progression of wound contraction/area and mean wound healing time. Results: Animals exposed to 2 J/cm2 showed significant (p<0.01) enhancement of wound contraction at day 5, day 9, day 12, day 14, day 16 and day 19 post irradiation compared to the controls. Treatment with laser at 2 J/cm2 resulted a significant (p<0.01) decrease in the healing time (19.83�0.38 days, post irradiation) compared to controls (25.92�0.8 days). The animals exposed to 2 J/cm2 immediately (0 hr) after wounding showed enhanced wound contraction when compared to control and other treatment schedules. Conclusion: The LLLT system designed and developed in this study has demonstrated optimum tissue regeneration for 2 J/cm2 He-Ne laser irradiation, applied immediately after the wounding compared to other post wounding treatment schedules by promoting wound contraction and thereby reducing mean healing time.

Item Type: Article
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Plastic Surgery
Depositing User: KMC Manipal
Date Deposited: 27 Jul 2012 08:49
Last Modified: 11 Dec 2015 09:24
URI: http://eprints.manipal.edu/id/eprint/76746

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