Autofluorescence of Normal, Benign, and Malignant Ovarian Tissues: A Pilot Study

Kamath, Sudha D and Bhat, Rani A and Ray, Satadru and Mahato, KK (2009) Autofluorescence of Normal, Benign, and Malignant Ovarian Tissues: A Pilot Study. Photomedicine and Laser Surgery, 27 (2). pp. 325-335.

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Objective: The objective of this study is to evaluate the efficacy of laser-induced fluorescence (LIF) data obtained at 325-nm pulsed laser excitation for the discrimination of normal, benign, and malignant ovarian tissues. Background Data: Several studies have reported that the autofluorescence technique has a high specificity and sensitivity for discrimination between diseased and non-diseased tissues of various cancers, and also has the advantages of being non-invasive and producing a real-time diagnosis. When using this technique on ovarian tissues in most of the previously reported studies, multivariate statistical tools were used and classification analyses were carried out. Materials and Methods: Autofluorescence spectra of normal, benign, and malignant ovarian tissues were recorded with 325-nm pulsed laser excitation in the spectral region from 350–600 nm in vitro. The spectral analysis for discrimination between the different types of tissues was carried out usingprincipal component analysis (PCA)-based non-parametric k-nearest neighbor (k-NN) analysis. Results: A total of 97 (34 normal, 33 benign, and 30 malignant) spectra were obtained from 22 subjects with normal, benign, and malignant tissues. The discrimination analysis of data using a PCA-based k-NN algorithm showed very good discrimination. The performance of the analysis was evaluated by calculating statistical parameters, specificity, sensitivity, and accuracy and were found to be 100%, 90.90%, and 94.2%, respectively. Conclusion:The results show that the discrimination of normal, benign, and malignant ovarian conditions can be achieved quite successfully using LIF.

Item Type: Article
Subjects: Life Sciences > MLSC Manipal
Medicine > KMC Manipal > Obstetrics & Gynaecology
Medicine > KMC Manipal > Surgical Oncology
Depositing User: KMC Manipal
Date Deposited: 27 Jun 2012 04:24
Last Modified: 07 Oct 2013 06:44

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