Mangiferin: A Xanthone Attenuates Mercury Chloride Induced Cytotoxicity and Genotoxicity in HepG2 Cells

Kaivalya, Mudholkar and Rao, Nageshwar B and Rao, Satish BS (2011) Mangiferin: A Xanthone Attenuates Mercury Chloride Induced Cytotoxicity and Genotoxicity in HepG2 Cells. Journal of Biochemical and Molecular Toxicology, 25 (2). pp. 108-116.

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Abstract

Mangiferin (MGN), a dietary Cglucosylxanthone present in Mangifera indica, is known to possess a spectrum of beneficial pharmacological properties. This study demonstrates antigenotoxic potential of MGN against mercuric chloride (HgCl2) induced genotoxicity in HepG2 cell line. Treatment of HepG2 cells with various concentrations of HgCl2 for 3 h caused a dose-dependent increase in micronuclei frequency and elevation in DNA strand breaks (olive tail moment and tail DNA). Pretreatment with MGN significantly ( p < 0.01) inhibited HgCl2- induced (20 μM for 30 h) DNA damage. An optimal antigenotoxic effect of MGN, both in micronuclei and comet assay, was observed at a concentration of 50 μM. Furthermore, HepG2 cells treated with various concentrations of HgCl2 resulted in a dose-dependent increase in the dichlorofluorescein fluorescence, indicating an increase in the generation of reactive oxygen species (ROS). However, MGN by itself failed to generate ROS at a concentration of 50 μM, whereas it could significantly decrease HgCl2-induced ROS. Our study clearly demonstrates that MGN pretreatment reduced the HgCl2-induced DNA damage in HepG2 cells, thus demonstrating the genoprotective potential of MGN, which is mediated mainly by the inhibition of oxidative stress.

Item Type: Article
Uncontrolled Keywords: Cytotoxicity; Genotoxicity; Mangiferin; Mercury Chloride; Micronuclei; Reactive Oxygen Species
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 01 Aug 2012 09:45
Last Modified: 01 Aug 2012 09:45
URI: http://eprints.manipal.edu/id/eprint/76901

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