Protective effect of Zingerone, a dietary compound against radiation induced genetic damage and apoptosis in human lymphocytes

Rao, Bhuvanagiri Nageshwar and Parampalli, Raghavendra Archana and Aithal, Balkudru Kiran and Rao, Satish BS (2011) Protective effect of Zingerone, a dietary compound against radiation induced genetic damage and apoptosis in human lymphocytes. European Journal of Pharmacology, 1 (3). pp. 59-66. ISSN 1879-0712

[img] PDF
Nagesh_EJP-67.pdf - Published Version
Restricted to Registered users only

Download (353kB) | Request a copy

Abstract

Zingerone a dietary compound was investigated for its ability to protect against radiation induced genotoxicity and apoptosis in human lymphocytes growing in vitro. The radiation antagonistic potential of zingerone was assessed by alkaline comet, cytokinesis-block micronucleus, apoptosis and reactive oxygen species inhibition assays. Treatment of lymphocytes with zingerone (10 μg/ml) prior exposure to 2 Gy gamma radiation resulted in a significant reduction of frequency of micronuclei as compared to the control set of cells evaluated by cytokinesis blocked micronucleus assay. Similarly, treatment of lymphocytes with zingerone prior to radiation exposure showed significant decrease in the DNA damage as assessed by comet parameters, such as percent tail DNA and Olive tail moment. Further, treatment with zingerone (10 μg/ml) before irradiation significantly decreased the percentage of apoptotic cells analyzed microscopically method and by DNA ladder assay. Similarly, the radiation induced reactive oxygen species levels were significantly (Pb0.01) inhibited by zingerone. Our study demonstrates the protective effect of zingerone against radiation induced DNA damage and antiapoptotic effect in human lymphocytes, whichmay be partly attributed to scavenging of radiation induced free radicals and also by the inhibition of radiation induced oxidative stress.

Item Type: Article
Uncontrolled Keywords: Apoptosis Genotoxicity Radiation Reactive oxygen species Zingerone
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 03 Aug 2012 06:53
Last Modified: 11 Dec 2015 09:20
URI: http://eprints.manipal.edu/id/eprint/76916

Actions (login required)

View Item View Item