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Mangiferin, a Dietary Xanthone Protects Against Mercury-Induced Toxicity in HepG2 Cells

Agarwala, Sobhika and Rao, Nageshwar B and Mudholkar, Kaivalya and Bhuwania, Ridhirama and Rao, Satish BS (2012) Mangiferin, a Dietary Xanthone Protects Against Mercury-Induced Toxicity in HepG2 Cells. Environmental Toxicology, 27 (2). pp. 117-127.

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Abstract

Mercury is one of the noxious heavy metal environmental toxicants and is a cause of concern for human exposure. Mangiferin (MGN), a glucosylxanthone found in Mangifera indica, reported to have a wide range of pharmacological properties. The objective of this study was to evaluate the cytoprotective potential of MGN, against mercury chloride (HgCl2) induced toxicity in HepG2 cell line. The cytoprotective effect of MGN on HgCl2 induced toxicity was assessed by colony formation assay, while antiapoptotic effect by fluorescence microscopy, flow cytometric DNA analysis, and DNA fragmentation pattern assays. Further, the cytoprotective effect of MGN against HgCl2 toxicity was assessed by using biochemical parameters like reduced glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT) by spectrophotometrically, mitochondrial membrane potential by flowcytometry and the changes in reactive oxygen species levels by DCFH-DA spectrofluoremetric analysis. A significant increase in the surviving fraction was observed with 50 lM of MGN administered two hours prior to various concentrations of HgCl2. Further, pretreatment of MGN significantly decreased the percentage of HgCl2 induced apoptotic cells. Similarly, the levels of ROS generated by the HgCl2 treatment were inhibited significantly (P \ 0.01) by MGN. MGN also significantly (P \ 0.01) inhibited the HgCl2 induced decrease in GSH, GST, SOD, and CAT levels at all the post incubation intervals. Our study demonstrated the cytoprotective potential of MGN, which may be attributed to quenching of the ROS generated in the cells due to oxidative stress induced by HgCl2, restoration of mitochondrial membrane potential and normalization of cellular antioxidant levels.

Item Type: Article
Uncontrolled Keywords: apoptosis; cytotoxicity; mangiferin; mercury chloride and reactive oxygen species
Subjects: Life Sciences > MLSC Manipal
Depositing User: KMC Manipal
Date Deposited: 03 Aug 2012 06:56
Last Modified: 26 Feb 2013 09:53
URI: http://eprints.manipal.edu/id/eprint/76918

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