Demonstration of pemphigus-specific immunofluorescence pattern by direct immunofluorescence of plucked hair

Rao, Raghavendra and Dasari, Kavitha and Shenoi, Shrutakirthi D and Balachandran, C (2009) Demonstration of pemphigus-specific immunofluorescence pattern by direct immunofluorescence of plucked hair. International Journal of Dermatology, 48 (11). pp. 1187-1189.

[img] PDF
DIF_hair.pdf - Published Version
Restricted to Registered users only

Download (178kB) | Request a copy

Abstract

Background:Direct immunofluorescence (DIF) of perilesional skin is the gold standard in the diagnosis of pemphigus. Since the outer root sheath (ORS) of anagen hair is structurally analogous to epidermal keratinocytes, pemphigus specific immunofluorescence patternmay be present in theORS. Methods Twenty consecutive patients of pemphigus were enrolled in the study, irrespective of any other inclusion or exclusion criteria.Hairswere plucked in a similar fashion to that of trichogram. Approximately 5 anagen hairswere selected processed and stained with fluorescein isothiocyanate (FITC) conjugates. DIF of perilesional skin and indirect immunofluorescence (IIF) were carried out simultaneously. DIF of hair was also done in equal numbers of controls with other dermatoses. Results Intercellular deposition of IgGwas seen in theORS of anagen hair in 85%of patients (n¼17). The testwas positive in all patients who had scalp lesions (n¼10); however, itwas also positive in 7 patients (77.7%) who did not have scalp lesions. The test was negative in 3 patients; two ofwhomwere elderly and had sparse scalp hair. The other patient only had mucosal lesion. The testwas negative in the control group. Conclusion DIF of hair is a simple, non-invasive test. In future, itmay alleviate the need for skin biopsies, in patients with pemphigus.

Item Type: Article
Subjects: Medicine > KMC Manipal > Dermatology
Depositing User: KMC Manipal
Date Deposited: 10 Aug 2012 09:23
Last Modified: 04 Nov 2016 09:22
URI: http://eprints.manipal.edu/id/eprint/76986

Actions (login required)

View Item View Item